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Labs & Technologies

Translation Factors Structural Biology Unit

Reveals the mechanisms of translation

Unit Leader
Takuhiro Ito  Ph.D.

Room N217, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa 230-0045, Japan
Tel: +81-45-503-9204

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Research Area

We plan to conduct studies on three dimensional structures of the translation factors and/or the ribosome, mainly by X-ray crystallography, focusing on the structural analysis of the large complexes that function in the eukaryotic translational system, in order to reveal the complicated mechanisms of eukaryotic translation.

Main Publications List

1

Crystal structure of eukaryotic translation initiation factor 2B

Kashiwagi K, Takahashi M, Nishimoto M, Hiyama TB, Higo T, Umehara T, Sakamoto K, Ito T, Yokoyama S
Nature, 351(7592), 122-125 (2016).
2

Expression, purification, and crystallization of Schizosaccharomyces pombe eIF2B

Kashiwagi K, Shigeta T, Imataka H, Ito T, Yokoyama S
J Struct Funct Genomics, 17(1), 33-38 (2016).
3

Structural basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD.

Ito T, Masuda I, Yoshida K, Goto-Ito S, Sekine S, Suh SW, Hou YM, Yokoyama S.
Proc Natl Acad Sci USA., 112(31), E4197-205 (2015).
4

RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing

Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Gntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, Muto Y.
Nat Struct Mol Biol, 21(9), 778-786 (2014).
5

Crystal structure of the eukaryotic translation initiation factor 2A from Schizosaccharomyces pombe

Kashiwagi K, Ito T, Yokoyama S.
J Struct Funct Genomics, 15(3), 125-130 (2013).
6

Differentiating analogous tRNA methyltransferases by fragments of the methyl donor.

Lahoud G, Goto-Ito S, Yoshida K, Ito T, Yokoyama S, Hou YM.
RNA, 17(7), 1236-1246 (2011).
8

Structure of nondiscriminating glutamyl-tRNA synthetase from Thermotoga maritima.

Ito T, Kiyasu N, Matsunaga R, Takahashi S, Yokoyama S.
Acta Crystallogr Sect D-Biol Crystallogr, 66(Pt7), 813-820 (2010).
9

Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation.

Goto-Ito S, Ito T, Kuratani M, Bessho Y, Yokoyama S.
Nat Struct Mol Biol, 16(10), 1109-1115 (2009).
10

Crystal structure of the alpha subunit of human translation initiation factor 2B.

Hiyama TB, Ito T, Imataka H, Yokoyama S.
J Mol Biol, 392(4), 937-951 (2009).

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